Determining Total Protein Content of Urine: Centrifugation Results in Precipitation of the Protein-Coomassie Brilliant Blue Dye Complex
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چکیده
منابع مشابه
Determining total protein content of urine: centrifugation results in precipitation of the protein-coomassie brilliant blue dye complex.
The Coomassie Brilliant Blue (CBB) protein dye-binding assay (1) has been favorably assessed for determining the total protein content of urine (2-4), although discrepancies have been noted in its response to different proteins (5) and sample dilution (6). It is commonly accepted that the proteinCBB dye complex is soluble under assay conditions, but our studies reveal loss of color yield after ...
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ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 1992
ISSN: 0009-9147,1530-8561
DOI: 10.1093/clinchem/38.6.1186